Environmental Engineering Reference
In-Depth Information
Ta b l e 6
Details of some studied pertaining to metabolic engineering carried out to enhance
fermentative H
2
production
Nature of genetic
modification
Microorganism
Comments
References
Inactivating hycA gene and
simultaneous
overexpression of the
formate hydrogen lyase
activator fhlA gene
Escherichia coli
Improved H
2
production
[138]
Knocking out lactate
dehydrogenase
Escherichia coli
35% improvement in H
2
production
[139]
Blocking the formation of
alcohol and some organic
acids using the proton
suicide technique with
NaBr and NaBrO3
Enterobacter
cloacae
Improved H
2
production
(3.4 mol H
2
/mole of glucose)
[140]
Knocking out ackinase
Clostridium
tyrobutyricum
ATCC 25755
Improved H
2
production
(2.61 mol H
2
/mole of
glucose)
[141]
Inactivation of
hycA
gene
Escherichia coli
HD 701
14 fold increase in H
2
production at lower glucose
concentration (100 mmol)
[142]
Overexpression of its own
hydA
gene encoding
[Fe]-hydrogenease
Clostridium
paraputrificum
M121
1.7 fold improvement in H
2
production (2.4 mol H
2
/mole
n-acetyl glucosamine)
[143]
Inactivation of acetate
kinase
Clostridium
tyrobutyricum
1.5 fold improvement in H
2
production (2.2 mol H
2
/mole
glucose)
[144]
Double mutant
Eneterobacter
aerogens
strain
AY2
2 fold improvement in H
2
production (1.2 mol H
2
/mole
glucose)
[145]
Knocking out formate
hydrogen lyase (FHL)
gene cluster hycABCDE
in chromosomal DNA
Enterobacter
aerogenes
IAM1183
Improved H
2
production (from
18.3 to 45.2%) and purity
(from 59 to 71%)
[146]
Altering expression of
formate hydrogen lyase
(FHL) by inactivating
repressor gene and
overexpressing the
activator gene
simultaneously
Escherichia coli
K12
4 fold improvement in H
2
production
[147]
from power generation, the MFC also demonstrated an enhanced substrate degrada-
tion rate along with good color and total dissolved solid (TDS) removal efficiency
compared to conventional anaerobic treatment [156]. MFCs can also utilize acid-
rich carbon effluents generated from acidogenic processes as primary substrate for
bioelectricity generation along with additional treatment efficiency.
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