Biology Reference
In-Depth Information
CHAPTER 3
Making and Using Calcium-Selective
Mini- and Microelectrodes
*Cardiovascular Research Centre CSIC-ICCC
Hospital de la Santa Creu i Sant Pau
Barcelona, Spain
†
Ricerca Biosciences SAS
Saint Germain sur l'Arbresle, France
‡
Department of Pharmacology
University of California, Davis,
Davis, California, USA
Abstract
I. Introduction
A. Main Characteristics of Ca
2
þ
-Selective Electrodes
II. Rationale
III. Methods
A. Preparation of Minielectrodes
B. Application of Minielectrodes
C. Preparation of Ca
2
þ
-Selective MEs
D. Application of Ca
2
þ
-Selective MEs
IV. Discussion
References
Abstract
Detection and measurement of intracellular calcium concentration ([Ca
2
þ
]
i
)
have relied on various methods, the popularity of which depends on their ease of
use and applicability to di
erent cell types. Historically, Ca
2
þ
-selective electrodes
have been used concomitantly with absorption indicators such as arsenazo-III,
but their interest has been eclipsed by the introduction of a large number of
fluorescent calcium probes with calcium sensitivities varying from the nanomolar
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