Biology Reference
In-Depth Information
Imaging mode
Bioluminescence
Bright-field
Fluorescence
A
Fiber optic
light guide
Bright-field
condenser
Electronic shutter
Halogen
lamp
Bioluminescent/
fluorescent sample
Electronic shutter
Motorized
XY stage
Mercury
arc lamp
Fiber optic light guide
Motorized
Objective turret
High NA
objective
Epifluorescent
filter set
B
Motorized
focus
Motorized
filter turret
Fluorescence
condenser
To microscope
interface (in CPU)
Tube
lens
Reducing
lens
Electronic
shutter
Shutter driver
Photon
imaging
software
Image
data
EMCCD
EMCCD
EMCCD
Multicore CPU
Microscope
interface
Water
chiller
To EMCCD interface (in CPU)
EMCCD interface
Dark box
Light from
halogen lamp
C
Motorized
focus
Epifluorescent
filter set
Light from
mercury
arc lamp
FL slider
Motorized
mirror
Fluorescence
condenser
CCD
CCD
CCD
To frame
grabber
(in CPU)
Shutter driver
To microscope
interface (in CPU)
RA-IPD
controller
Tube
lens
Reducing
lens
Electronic
shutter
Photon
imaging
software
Image
data
RA-IPD
RA-IPD
RA-IPD
Multicore CPU
Microscope interface
RA-IPD interface
Frame grabber
*
Water
chiller
To RA-IPD interface (in CPU) and
RA-IPD controller
*
Only required for RA-IPD
Dark box
Fig. 4
Schematic representation of luminescence imaging systems based around a modified EMCCD
and an RA-IPD, which can be used to acquire bright-field and fluorescence imaging information as well
as collect bioluminescence data. (A) Components that are common to both the EMCCD- and RA-IPD-
based systems. (B and C) Components that are specific for the (B) EMCCD- and (C) RA-IPD-based
