Biology Reference
In-Depth Information
A
Dark box
IR
camera
Zoom
lens
Monitor
Count plot
IR
image
Feed-
through
IR ring light
Sample
Temperature-
controlled
stage
Manual
PMT
shutter
PMT system
controller
IR
blocking
filter
Computer
Shutter
PMT
1252
HV/amplifier/
discriminator
Door
switch
B
C
Perfusion
Perfusion
Mirror
ICCD
Perfusion
chamber
Light-proof
chamber
Cells
Glass
window
PMT1
Filter
Lens
Dichroic
mirror
(585 DCXR)
IR
lighting
IR-sensitive
camera
D535-50
Beam-
splitter
D630-60
Lens
PMT2
Sample stage
(large field of view)
Fig. 2
Schematic representations of three recently developed luminescence detection systems. (A)
A system that combines a PMT with an IR light source and an IR-sensitive camera. This enables both
single photon detection in the visible light spectrum and monitoring the sample continuously in real-
time using IR light. The system was designed and built by Science Wares, Inc., (Falmouth, MA). (B)
A two-channel luminometer, which was designed to simultaneously collect temporal Ca
2
รพ
signaling
