The Use of Aequorins to Record and Visualize Ca Dynamics: From Subcellular Microdomains to Whole Organisms - Methods in Cell Biology - page 271

Kingdom: Protista
Dictyostelium discoidium (Slime mold) Cells were incubated with coelenterazine solution to a final concentration of

50 m M for 24 h at r.t. in the dark

Cubitt et al. (1995)

Kingdom: Monera
Escherichia coli Cells, diluted in 100 mMKCl, 1 mMMgCl 2 , Tris-HCl, pH 7.5, were incubated

with coelenterazine (final concentration of 2.5 m M) o/n at r.t. in the dark

Knight et al. (1991b)

Bacillus subtilis Bacteria were incubated in TS medium containing 20 m g/ml kanamycin and

2.5 m M coelenterazine h for 1 h in the dark at r.t.

Herbaud et al. (1998)

Anabaena sp. Cells were incubated with coelenterazine (to a final concentration of 2.5 m M)

for 4 h in the dark

Torrecilla et al. (2000)

Tissue Culture Cells
H4-IIE cells Cells were incubated with 5 m M coelenterazine in Krebs-Ringer modified

bu

Chan et al. (2004)

V er containing 1 mM EGTA for 1 h at r.t.

Neuro2A cells Cells were incubated with 5 m M coelenterazine, 10 mM b -mercaptoethanol Baubet et al. (2000)

and 4 mM EDTA in PBS for 2-4 h at 4 C

Cells were incubated with 2.5 m M coelenterazine for 6-8 h at 37 C

HeLa cells Brini et al. (1993)

Cells were incubated with 5 m M coelenterazine-n for 2 h at 37 C

Jurkat cells Narayanan et al. (2003)

COS7 cells Cells in suspension were incubated with 2.5 m M coelenterazine in DMEM o/n

at 4 C

Kendall et al. (1994)

r.t. is room temperature; o/n is overnight.