Biology Reference
In-Depth Information
Table III
Examples of the some of the reported coelenterazine loading protocols
Species
Coelenterazine loading protocol reported
References
Kingdom: Animalia
Mus musculis (Mouse)
Native coelenterazine was introduced into adult mice (at 4 mg/kg) by tail-vein
injection and into new-born mice (at 2-4 m g/g) by intraperitoneal injection.
Light emission was recorded immediately
Rogers et al. (2007)
Minced tissues or cells of tissues were incubated with 0.2 ml RPMI 1640
containing 10 m M coelenterazine at 37 C for 5 h
Yamano et al. (2007)
Danio rerio (Zebrafish)
Embryos that had been dechorionated at the 64-cell stage were incubated with
50 m M f-coelenterazine, prepared in 30% Danieau's solution
Cheung et al. (2006)
Xenopus laevis (African clawed frog)
Oocytes were incubated in 2.5 m M coelenterazine in a medium containing
5mMb-mercaptoethanol
Daguzan et al. (1995)
Drosophila melanogaster (Fruit fly)
Malpighian tubules from 4 to 14-day old adults were incubated in Schneider's
medium containing 2.5 m M coelenterazine for 4-6 h in the dark
Rosay et al. (1997)
Exposed fly brains were incubated in fly ringers solution containing 5 m M
native coelenterazine for > 1 h at r.t.
Martin et al. (2007)
Kingdom: Plantae
Nicotiana plumbaginifolia (Tobacco)
Seedlings were floated on water containing 2.5 m M coelenterazine o/n at r.t. in
the dark
Knight et al. (1991a, 1996)
Arabidopsis thaliana
Seedlings were submerged in 10 m M coelenterazine (which had been dissolved
in ethanol) in dH 2 O for 7.5 h at r.t. in the dark
Sedbrook et al. (1996)
Seedlings were floated on water containing 2.5 m M coelenterazine o/n at r.t. in
the dark
Knight et al. (1996)
Solanum tuberosum (Potato)
Plants were incubated in 5 m M hcp coelenterazine for 8 h
Fisahn et al. (2004)
Physcomitrella patens (Moss)
Ground up moss tissue was incubated in 0.5 MNaCl, 5 mMmercaptoethanol,
5 mM EDTA, 0.1% gelatin, 10 mM Tris-HCl pH 7.4 containing 2.5 m M
coelenterazine for 6 h or o/n
Russell et al. (1996)
Kingdom: Fungi
Neurospora crassa / Aspergillus niger /
Aspergillus awamori
Vogel's medium containing 2.5 m M coelenterazine (prepared in methanol) was
inoculated with 1
Nelson et al. (2004)
10 5 spores/ml. Inoculated medium was incubated for
24 h at 30 C in the dark
Phyllosticta ampelicida
2.5 m M coelenterazine was pipetted over colonies growing in 1/2
potato
Shaw et al. (2001)
dextrose agar and incubated for 4 h
Schizosaccharomyces pombe
Cells were incubated in EMM medium containing 20 m M coelenterazine for
4 h at 30 C
Deng et al. (2006)
Saccharomyces cerevisiae
0.1 volume of 590 m M coelenterazine (prepared in methanol) was added to
25-30 m l yeast culture and incubated for 20 min at r.t.
Batiza et al. (1996)
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