Biology Reference
In-Depth Information
Table II
Examples where apoaequorin or apoaequorin-BRET complexes have been targeted to the cytosol of cells in culture or to
distinct intracellular organelles either in cells in culture or intact organisms
Species (common
name
and/or scientific
name)
Cytosolic expression of
apoaequorin or
apoaequorin-BRET
Cell type
Organelle(s) targeted
Comments
References
Kingdom: Animalia
Human (Homo
sapiens)
ECV304 (umbilical
vein endothelial cells)
Mitochondria
Transient expression
Lawrie et al. (1996)
✓
1
cell cultures
(Transient
expression)
Diploid fibroblasts
(used indo 1-AM
and fura 2-AM
Mitochondria
Padua et al. (1998)
HEK-293 cells (embry-
onic kidney cell line)
-
Some transient
and some stable
expression
Sheu et al. (1993)
✓
✓
-
Stable expression
Button and
Brownstein (1993)
✓
Nucleus
HSV gene
transfer vector
Chamero et al. (2002)
✓
ER lumen,
mitochondrial
matrix
Transient expression
Brini et al. (2005)
Manjarr
´
s et al. (2008)
HEK 293T cells
✓
Nucleus,
mitochondria, ER
Expression of GA
and RA via HSV
gene transfer
vector
HeLa (immortalized
epithelial cell line)
Nucleus
Stable expression
Brini et al. (1993)
✓
(used Fura-2)
ER lumen
Transient and stable
expression
Montero et al. (1995)
1
cell cultures
(HSV gene
transfer vector)
(used fura 2)
ER
Alonso et al. (1998)
Mitochondria,
Golgi, ER
Transient expression
Pinton et al. (2000)
✓
(used indo-1)
Golgi, ER
Stable expression
Missiaen et al. (2004)
1
cell cultures
(Transient
expression)
Normal adult and
Hailey-Hailey disease
keratinocytes
(used proton-induced
X-ray emission)
Golgi
Behne et al. (2003)
Jurkat cells (immorta-
(used indo PE3 (AM))
ER
Transient expression
Narayanan et al. (2003)
