The Use of Aequorins to Record and Visualize Ca Dynamics: From Subcellular Microdomains to Whole Organisms - Methods in Cell Biology - page 263

A

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Fig. 1 Examples of the spatial patterns of Ca 2 þ signals generated by whole organisms, or by specific
tissues or subcellular organelles within intact organisms, where aequorin or GFP-aequorin were
genetically expressed. (A) Newborn mice stably expressing GFP-aequorin targeted to the mitochondrial
matrix were injected intraperitoneally with native coelenterazine and bioluminescence activity was
recorded with the animals un-restrained and freely moving. These are consecutive video images on to
which have been superimposed the corresponding bioluminescence images. Each panel represents 2 s of
accumulated light. Scale bar is 5 mm. Reproduced with permission, from Rogers et al. (2007) . (B) An 18-
somite stage (i.e., 18 h postfertilization (hpf)) zebrafish embryo that was injected with apoaequorin
mRNA at the one-cell stage to transiently express apoaequorin throughout the whole embryo, and then