Biology Reference
In-Depth Information
VI. Summary
The patch clamp technique provides quantitative records of calcium channel
activity in the plasma membrane at the molecular level in real time in situ.
However, studying calcium channel function and regulation under physiologi-
cal conditions in metabolically intact cells requires more demanding approaches
than the conventional whole-cell recording through ruptured patches on dia-
lyzed cells. Whole-cell recordings through antibiotic-perforated patches and
single-channel recordings from cell-attached patches require additional e
ort
and attention to detail, but are currently unrivaled in their precision and
detailed view of calcium channel function and physiological regulation of
gating.
V
Acknowledgments
Our studies have been supported by the NIH intramural program at NIEHS through Grant Z01-
ES080043. We also thank Sue Edelstein for drawing Figures 1 & 2.
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