Genetically Encoded Probes for Measurement of Intracellular Calcium - Methods in Cell Biology - page 179

Persechini, A., Lynch, J. A., and Romoser, V. A. (1997). Novel fluorescent indicator proteins for
monitoring free intracellular Ca2

209-216.
Pologruto, T. A., Yasuda, R., and Svoboda, K. (2004). Monitoring neural activity and [Ca 2 þ ] with
genetically encoded Ca 2 þ indicators. J. Neurosci.

þ . Cell Calcium 22,

9572-9579.
Pozzan, T., and Rudolf, R. (2009). Measurements of mitochondrial calcium in vivo. Biochim. Biophys.
Acta

24,

1787, 1317-1323.

Rei erential regulation of active zone density

during long-term strengthening of Drosophila neuromuscular junctions. J. Neurosci.

V , R. F., Thiel, P. R., and Schuster, C. M. (2002). Di V

9399-9409.
Rei V , D. F., Ihring, A., Guerrero, G., Isaco V Ehud, Y., Joesch, M., Nakai, J., and Borst, A. (2005). In
vivo performance of genetically encoded indicators of neural activity in flies. J. Neurosci.

22,

25,

4766-4778.
Rekas, A., Alattia, J.-R., Nagai, T., Miyawaki, A., and Ikura, M. (2002). Crystal structure of venus, a
yellow fluorescent protein with improved maturation and reduced environmental sensitivity. J. Biol.
Chem. 277, 50573-50578.
Rizzuto, R., Simpson, A. W. M., Brini, M., and Pozzan, T. (1992). Rapid changes of mitochondrial
Ca 2 þ revealed by specifically targeted recombinant aequorin. Nature 358, 325-327.
Robert, V., Gurlini, P., Tosello, V., Nagai, T., Miyawaki, A., Di Lisa, F., and Pozzan, T. (2001). Beat-
to-beat oscillations of mitochondrial [Ca2 þ ] in cardiac cells. EMBO J. 20, 4998-5007.
Romoser, V. A., Hinkle, P. M., and Persechini, A. (1997). Detection in living cells of Ca2 þ -dependent
changes in the fluorescence emission of an indicator composed of two green fluorescent protein
variants linked by a calmodulin-binding sequence. A new class of fluorescent indicators. J. Biol.
Chem. 272, 13270-13274.
Rudolf, R., Mongillo, M., Magalhaes Paulo, J., and Pozzan, T. (2004). In vivo monitoring of Ca(2 þ )
uptake into mitochondria of mouse skeletal muscle during contraction. J. Cell Biol. 166, 527-536.
Shimozono, S., Fukano, T., Nagai, T., Kirino, Y., Mizuno, H., and Miyawaki, A. (2002). Confocal
imaging of subcellular Ca2

þ

concentrations using a dual-excitation ratiometric indicator based on
green fluorescent protein. Sci. STKE

2002,

pl4.
Shimozono, S., Fukano, T., Kimura, K. D., Mori, I., Kirino, Y., and Miyawaki, A. (2004). Slow Ca 2 þ
dynamics in pharyngeal muscles in Caenorhabditis elegans during fast pumping. EMBORep.

521-526.
Silver, R. A., Whitaker, M., and Bolsover, S. R. (1992). Intracellular ion imaging using fluorescent dyes:
Artefacts and limits to resolution. Pflugers Arch.

5,

595-602.
Solovyova, N., and Verkhratsky, A. (2002). Monitoring of free calcium in the neuronal endoplasmic
reticulum: An overview of modern approaches. J. Neurosci. Methods

420,

1-12.
Souslova, E. A., Belousov, V. V., Lock, J. G., Stromblad, S., Kasparov, S., Bolshakov, A. P.,
Pinelis, V. G., Labas, Y. A., Lukyanov, S., Mayr, L. M., and Chudakov, D. M. (2007). Single
fluorescent protein-based Ca2

122,

37.
Suzuki, H., Kerr, R., Bianchi, L., Frokjaer-Jensen, C., Slone, D., Xue, J., Gerstbrein, B., Driscoll, M.,
and Schafer, W. R. (2003). In vivo imaging of C. elegans mechanosensory neurons demonstrates a
specific role for the MEC-4 channel in the process of gentle touch sensation. Neuron 39, 1005-1017.
Tallini, Y. N., Ohkura, M., Choi, B.-R., Ji, G., Imoto, K., Doran, R., Lee, J., Plan, P., Wilson, J.,
Xin, H.-B., Sanbe, A., Gulick, J., et al . (2006). Imaging cellular signals in the heart in vivo: Cardiac
expression of the high-signal Ca2 þ indicator GCaMP2. Proc. Natl. Acad. Sci. USA 103, 4753-4758.
Tian, L., Hires, S. A., Mao, T., Huber, D., Chiappe, M. E., Chalasani Sreekanth, H., Petreanu, L.,
Akerboom, J., McKinney, S. A., Schreiter, E. R., Bargmann, C. I., Jayaraman, V.,, et al . (2009).
Imaging neural activity in worms, flies and mice with improved GCaMP calcium indicators. Nat.
Methods 6, 875-881.
Truong, K., Sawano, A., Mizuno, H., Hama, H., Tong, K. I., Mal, T. K., Miyawaki, A., and Ikura, M.
(2001). FRET-based in vivo Ca2 þ imaging by a new calmodulin-GFP fusion molecule. Nat. Struct.
Biol. 8, 1069-1073.
Tsien, R. Y. (1998). The green fluorescent protein. Ann. Rev. Biochem. 67, 509-544.
Tsien, R. Y. (2010). The 2009 Lindau Nobel Laureate Meeting: Roger Y. Tsien, Chemistry 2008. JoVE
35. http://www.jove.com/index/Details.stp?ID=1575 , doi: 10.3791/1575.

þ sensors with increased dynamic range. BMC Biotechnol. 7,