Biology Reference
In-Depth Information
[Ca
2+
]
0
=75nM
9
9
8
8
7
7
[Ca
2+
]
[Ca
2+
]
0
6
6
5
5
F
F
0
4
4
3
3
2
2
1
1
0
0
1
2
3
4
5
6
7
8
9
10
[Ca
2+
]/[Ca
2+
]
0
Fig. 14
Specific example illustrating that a relative change in fluorescence (F/F
0
) of the indicator,
Fluo-4, does not accurately reflect the true relative change in [Ca
2
þ
] ([Ca
2
þ
]/[Ca
2
þ
]
0
).
A
B
Fig. 15
(A) Structures of Rhod-2 AM (bromide salt), as well as TMRM and TMRE (percholorate
salts), two dyes that accumulate preferentially into mitochondria. Note that in each case, the
dye molecule bears a permanent positive charge. (B) A series of related resonance structures
showing that the positive charge can be located on di
V
erent atoms in the molecule; that is, the charge
is delocalized.
