Biology Reference
In-Depth Information
A
5
4.5
Prediction
4
Δ
H =0
3.5
Data
3
2.5
2
1
8
15
22
29
36
Temperature (C)
B
7
6
Prediction
5
4
3
Data
2
1
0
0.1
0.15
0.2
0.25
0.3
Ionic strength (M)
Fig. 4 BAPTA apparent Ca 2 þ a Y nity (K 0 Ca ) is influenced by temperature (A) and ionic strength (B).
The experimental data is from Harrison and Bers (1987) at pH 7.00 and 0.19 M ionic strength (A) and at
pH 7.00 and 22 C (B). Predicted values are based on the temperature and ionic strength corrections
described in the text.
we do not want to belabor the equations, it may be useful for some readers if we lay
out some of the basics. If you are not interested in the equations, you can ignore
this section and the next (and still use the programs as more of a black box). We
hope we have accounted for things as well as possible.
In the sections above, we used K d to talk about Ca 2 þ a
nity. That K d was the
apparent overall dissociation constant, which we will get back to below (see
Eq. (5) ). It is more traditional to set out the mathematical expressions starting
with the simple definition of the Ca 2 þ association constant K Ca
Y
K Ca ¼ ½
CaR
ð1Þ
½
Ca
½
R
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