Environmental Engineering Reference
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Fig. 8. Effect of dissolved oxygen (DO) on different physiological responses of L. santolla
juveniles. Pc: critical point of aerobic metabolism shift; LDH: Lactate dehydrogenase; Prot:
protein concentration; Oxy-Hc: oxygenated hemocyanin; Glyc: glycogen; Chol: cholesterol;
AG: acylglycerids; Gluc: glucose.
concentration. These responses to reduced DO were complemented with anaerobic
metabolism evidenced by the increase in the activity of gill-LDH and a consequent increase
of Lactate concentration. A reduction in nutritional compounds in the hepatopancreas was
interpreted as a consequence of a notorious reduction of food ingestion, and as an additional
consequence of hypoxic conditions (the ingestion was affected severely and showed a linear
relationship to dissolved oxygen (DO) between 2 and 13 kPa: 0.59 and 1.92 mg Dry weight
h-1 ind-1, respectively). Severe chronic hypoxia (1 mgO2*l -1 or 2 kPa for 10 days) led to an
unsustainable reduction in the aerobic and anaerobic activity, where a reduction of the gill-
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