DNA Microarray Technology - DNA Microarray Technology and Data Analysis in Cancer Research - page 5

Fig. 1.3. DNA microarray analysis of gene expression profiles in pre-B leukemic
cells expressing P190, P210, or P230 BCR-ABL. Total RNA was isolated from the
parental ENU pre-B cell line and the same cell line expressing P190, P210, or P230. A total
of 24 000 genes were measured using Affymetrix GeneChip, and the data were analyzed
by F2 test. Expression of 2086 genes in BCR-ABL-expressing cells was found to be
significantly different from that for the reference (parental cell control) (α<0.05). The
scientific background of the pre-B leukemic cells expressing P190, P210, or P230 BCR-
ABL will be introduced in Chapter 2.
difficult technical problem in microarray studies, the current approach may
have done its best to find the most appropriate controls available. Some of
the findings obtained from this type of studies are real and useful, although
some genes that play critical roles in tumor formation might not be
detected due to the inappropriate controls used. A better way to improve
this situation is to isolate subpopulations of tumor cells with antibodies rec-
ognizing phenotypical cell surface markers. This approach can be taken
much more easily when studying human blood cancer cells. However, the