DNA Microarray Technology - DNA Microarray Technology and Data Analysis in Cancer Research - page 2

Fig. 1.1. Activation of signaling pathways at different levels in cells that are stimu-
lated by growth factors. Binding of growth factors to the cell surface receptor leads to
activation of intracellular downstream known (solid lines) and unknown (broken lines)
signaling molecules (colored blocks). The end result of this activation is to turn on the
transcription of genes and ultimately the translation of mRNA into proteins that promote
or inhibit cell growth. Measurement of synthesis of either mRNA or protein will reflect the
function of a gene.
first described in 2000 (Adessi et al ., 2000); and (5) transcription on chip,
which shows the transcription of a complete gene into mRNA on the chip
(Steffen et al ., 2005). In this topic, we focus on gene expression analysis
using DNA microarray technology.
1.1. Experimental Procedure
The procedure of a DNA microarray experiment includes multiple steps
from sample preparation to data analysis (Fig. 1.2), among which