Environmental Engineering Reference
In-Depth Information
Decrease in the
1 globulin zone can be due to hepatocellular insufficiency,
malnutrition or protein loss, congenital deficiency of
α
α
1 anti trypsin whereas
increase is mainly seen in inflammatory disorders.
Conclusions
Gel electrophoresis made significant advancement in protein separation and
identification field. 2-D PAGE is a most commonly used technique in prote-
omic studies besides its long list of advances. Although, 2D-PAGE, like any
fractionation scheme, has limitations, however the potential to explore
though 2D PAGE is much more higher compared to other gel electrophoresis
techniques. Literature confirms successful use of 2D PAGE in various phar-
maceutical applications, e.g. identification of proteins, separation, microRNA
research, vaccine and antibiotic development area. Various laboratories con-
firmed potential of 2D-PAGE for predicting targeting potential of
nanoparticles. It is an essential technique for the characterisation of proteins
2-D PAGE holds promise in several pharma applications and, with no doubts
it will be a method of choice in coming decades.
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