Environmental Engineering Reference
In-Depth Information
Chapter 15
Multi-dimensional Electrophoresis: The
March in Pharma Applications
Ranjita Shegokar and Sampada Sawant
Abstract Electrophoresis is widely used in pharmaceutical field for multiple
applications. It is mainly employed for protein separation and identification. The
information obtained on protein content and composition from multiple gel elec-
trophoresis provides basic knowledge required for drug design, biomarker, vaccine,
and antibiotic research. Furthermore, combination with advance analytical tech-
niques like MALDI-TOF, mass spectroscopy provides added advantages in
analysing protien samples. Electrophoresis by single and multiple dimensional
methods is becoming very popular in nanoparticle research to identify protein
corona around particle which can predict organ targeting when used in vivo. This
technique can also be used to detect toxicity potential of nanoparticles and gener-
ated oxidative stress. Multi-dimensional electrophoresis has already been used in
conventional pharmaceutical application and now is marching to nanoparticle
and/drug delivery research area.
List of Abbreviations
1-DE
1-Dimensional electrophoresis
2-DE
2-Dimensional electrophoresis
2D-DIGE
2-Dimensional differential in-gel electrophoresis
CE
Capillary electrophoresis
CNTs
Carbon Nano Tubes
CB
Carbon black
CSF
Cerebrospinal Fluid
IEF
Immunofixation
IEF
Isoelectric focussing
NEPHGE
Nonequilibrium pH gel electrophoresis
PAGE
Polyacrylamide gel electrophoresis
pI
Isoelectric point
