Environmental Engineering Reference
In-Depth Information
13.4.3 Ecotoxicology Applications
Farkas et al. [
24
] utilized NTA with two CCD cameras to determine the particle-
size distribution of Ag nanoparticles and Au nanoparticles dispersed in different
growth media for studying the ecotoxicological effects of these particles on
rainbow-trout hepatocytes.
Van Hoecke et al. [
25
] utilized NTA to determine the mode and the mean size of
cerium-dioxide nanoparticles in a study to determine for alga Pseudokirchneriella
subcapitata ecotoxicity toward green, two crustaceans (
Daphnia magna
and
Thamnocephalus platyurus
), and embryonic development of
Danio rerio
.
13.4.4 Microbiota
Synthesis of silver nanoparticles (Ag NPs) by the leaf extract of
Murraya koenigii
(Indian curry leaf tree) is reported in the study of Bondea et al. [
26
]. The Ag
nanoparticles thus obtained demonstrated remarkable antibacterial activity against
three human pathogenic bacteria when used in combination with commercially
available antibiotics.
Besinis et al. [
27
] investigated the toxicity of silver (Ag), titanium dioxide, and
silica nanoparticles (NPs) against the oral pathogenic species of
Streptococcus
mutants, compared to the routine disinfectant, chlorhexidine. The bacteria were
assessed using the minimum inhibitory concentration assay for growth, fluorescent
staining for live/dead cells, and measurements of lactate. Overall, Ag nanoparticles
were the best disinfectant and performed better than chlorhexidine.
13.4.5 Virology
In the study [
28
], Kramberger et al. used NanoSight LM10 with Nanoparticle
tracking analysis (NTA) software in order to evaluate the quantification of latex
particles, adenovirus 5, and influenza virus. NTA analysis was used to quantify
chromatographic fractions of adenovirus and influenza virus after purification on a
CIM monolithic column. The results demonstrated that nanoparticle tracking anal-
ysis is a method for fast estimation of virus concentration in different samples and it
can provide a better insight into the sample status.
Conclusions
NTA proved to be very suitable for analyzing nanoparticles. This technique is
suitable for analyzing protein aggregates and the sample preparation does not
