Environmental Engineering Reference
In-Depth Information
to stem cells with minimum toxicity offers advantage over viral and nonviral
delivery devices, providing a new arena in the field of cell-based therapeutics and
serves as a promising tool for stem cell therapy. Noncovalently modified polyplexes
with lysinylated and histidylated CHOL lipids have been reported for their
improved efficiency and reduced toxicity for gene delivery in bone marrow stem
cells [ 19 ]. Nanoformulation with either PEG-lipid membrane or PEG-Polylactic
acid, PEG-PLA polymer membrane was being employed in designing of artificial
red blood cells (RBC) [ 77 ]. PEG-grafted polyethylenimine (PEG-PEI) with easy
construction, better transfection efficiency, and capability of potentially targeting
modification have been reported to be promising tools in gene delivery to mesen-
chymal stem cells (MSCs) [ 78 ].
The cell proliferation and metabolic activity of rBMSCs were highly improved
by the use of three stable lecithins (soya, rapeseed, salmon) liposomes than other
phospholipids liposomes. These nanoliposomes used in pharmaceutics have better
bioefficiency as drug delivery systems for tissue engineering [ 19 ].
The characteristics of veno-occlusive disease in pediatric hematopoietic stem
cell (HSC) transplant recipients, their prophylactic regimen, risk factors, treatment
modalities on severity and outcome were evaluated using liposomal amphotericin B
(LAmB), vancomycin treatment as compared to oral busulphan use. LAmB vanco-
mycin treatment and total parenteral nutrition played a fair role in low incidence of
pediatric veno-occlusive disease in individuals with high-risk features [ 79 ].
Liposome protamine/DNA lipoplex (LPD) assembled from CLP and an anionic
complex of protamine, DNA to target various peptides (VTAMEPGQ) that can
home to rat mesenchymal stem cells (rMSCs). The transfection action of LPD
was executed by the synergetic effect of rMSC-targeting peptide and nuclear
localization signal (NLS) peptide for LPD for internalization inside the cells,
leading to increased gene expression, guide sleeping Beauty (SB) transposon
into nuclei with no obvious cell toxicity and influences the differentiation potential
of rMSCs. Therefore, for promising nonviral gene delivery vector in stem cell
therapy, the integration of SB transposon through LPD system is quite
effective [ 80 ].
Mouse induced pluripotent stem (iPS) cell-derived Flk-1(+) cells were incubated
with magnetic nanoparticle-containing liposomes (MCLs) and these cells were
mixed with diluted extracellular matrix (ECM) to accelerate revascularization of
ischemic hind limbs. This novel magnetite tissue engineering technology using
MCLs represents a promising new modality for therapeutic angiogenesis [ 81 ].
The differentiation of fetal liver stem/progenitor cells (FLSPCs) into hepatocyte-
like cells for in vivo therapeutic investigation, treatment of FLSPCs with
Hepatocyte growth factors (HGF) with di-methyl sulfoxide (DMSO) for
liposome-mediated transfection, GFP gene was introduced into the system.
The self-renewal property of FLSPCs in soft agar culture and effectively differen-
tiation into hepatocyte-like cells was mediated by GFP traced liposome-mediated
transfection continued over successive generations [ 82 ].
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