Multidynamic Liposomes in Nanomedicine: Technology, Biology, Applications, and Disease Targeting - Nanoparticles' Promises and Risks - page 165

a

b

Clean and
depyrogenize all
equipment,
pre-filter and
ultrafilter all
solutions

Lipids
Cholesterol
Lecithin

Dissolve in organic
solvent

Lipid solubilised in organic solvent

Inject Drying

Diafilter Hydrate

Multilamellar vesicle

Down size

Exchange of external buffer

Loading

Remove free drug

Pre-filter

Sterile filter

Fill vial,lyophilise, seal, label and packaged aseptically

Fig. 9.1 (a) The different methods employed for preparation of liposomes are tabulated sche-
matically [ 5 - 9 ]. (b) Steps of liposome preparations represented in a flow diagram, starting with the
mixture of lipids and terminating in packaging of liposomes [ 5 - 9 ]