Environmental Engineering Reference
In-Depth Information
Fig. 8.13 Fragmentation spectrum obtained by automated chip-based (-) nanoESI QTOF MS/MS
derived by using NeuAc
2
Gal
3
GlcNAc
2
GalNAcSer detected as a doubly charged ion at
m/z
890.32
across the 25-40 eV collision energy range.
Inset
: Fragmentation scheme of NeuAc
2
Gal
3
Glc-
NAc
2
GalNAc-Ser. Reprinted with permission from [
67
]
100 robot
with the quadrupole time-of-flight MS for the glycopeptides screening in the urine
of a Schindler disease patient was reported in two studies [
67
,
68
], in one of them
the platform being also off-line coupled with capillary electrophoresis separation
technique [
68
].
NanoMate QTOF MS methodology was found advantageous for discovery of
novel carbohydrate species in complex biological samples due to the increased
sensitivity, reproducibility, ionization efficiency and by the ability to generate a
sustained and constant electrospray.
An efficient and versatile method for separation of complex mixtures obtained
from biological sources is represented by capillary electrophoresis (CE). The
compatibility of the same platform described above has been tested in conjunction
The combination of fully automated chip-based nanoESI NanoMate
™
