Environmental Engineering Reference
In-Depth Information
Fig. 8.11 Automated (-) chipESI FTICR MS of a fraction from the urine of a patient suffering
from Schindler disease. Sample concentration: 5 pmol
l
1
, in methanol. Number of scans: 150.
Average sample consumption for MS experiment: 2.5 pmol.
Inset table
: Ions detected and
identified with a mass accuracy below 12 ppm in the mixture of
O
-glycosylated peptides at
5 pmol
μ
l
1
. Reprinted with permission from [
66
]
μ
was for 0.5 pmol
μ
l
1
. Hence as compared with previously reported data on a similar
glycopeptide mixture obtained by regular capillary-based (-) nanoESI FTICR MS
[
69
], the NanoMate system was found to exhibit a superior sensitivity, a higher
ionization efficiency of even less abundant glycopeptide species and a preferential
formation of multiply charged ions. A considerable number of triply charged ions
could be formed and detected with a high S/N ratio, whereas, by capillary-based (-)
nanoESI FTICR MS, no triply charged glycopeptide ions were formed/detected.
Additionally, four new components, not identified by any other method before
were detected by this novel approach and identified under a high mass accuracy.
For the identification of potential diagnostic marker components, the
Ty
mixture
of glycopeptides extracted from the urine of a healthy control person was further
investigated by (-) nanoESI chip FTICR MS under the same ionization/detection
conditions as those from patient urine analysis.
If in patient urine glycoconjugate species expressing saccharide chain lengths up
to undecasaccharide have been identified, in healthy control urine, besides their
