Environmental Engineering Reference
In-Depth Information
Fig. 7.5 SEM images of MSCs and SK-BR-3 cells before and after the treatment with colloidal
suspensions: (a) control (untreated) MSCs, (b) MSCs treated with suspension 1F, (c) MSCs treated
with suspension 2F, (d) control (untreated) SK-BR-3 cells, (e) SK-BR-3 cells treated with
suspension 1F, (f) SK-BR-3 cells treated with suspension 2F
in morphological appearance, while the magnetite nanoparticles are revealed cov-
ering the cell surface (Fig.
7.5c
).
Morphological characteristics of SK-BR-3 cells are depicted in Fig.
7.5d
, show-
ing the round shape, small diameter and cluster-like growth of this cellular type
in vitro, which are forming more like a network of cellular elongations and contact
points, with increased deposition of extracellular matrix within the resulted mesh.
SK-BR-3 tumor cells presented a very unusual behavior when left in contact with
fluid 1F (deriving from combustion synthesized nanoparticles) for 48 h, extruding
the nucleus, so that the cells were enucleated (Fig.
7.5e
)—which is a very rare
phenomenon.
When fluid 2F (deriving from co-precipitated nanoparticles) was added on these
tumor cells, they developed thicker elongations and anchorage structures, long, and
less interconnected with the surrounding cells (Fig.
7.5f
).
Transmission electron microscopy was performed on both cellular types, adher-
ent on cell culture inserts and suspension cells. Ultrastructural description of MSCs
focuses on segmented nucleus, multiple elongations, numerous mitochondria and
lysosomes, rare endoplasmic reticulum and intracytoplasmic vacuoles (Fig.
7.6a, b
).
SK-BR-3 tumor cells are characterized by large nucleus, multiple lipid vacuoles,
well represented endoplasmic reticulum distributed along the entire cytoplasm,
polyribosomes and mitochondria, showing intense metabolic processes these cells
are involved in (Fig.
7.6c, d
). Addition of fluid 2F (deriving from co-precipitated
nanoparticles) on SK-BR-3 cells determined some morphological changes, induc-
ing accumulation of MNPs at the cytoplasmic level, in large clusters (Fig.
7.7a, b
),
