IMGT-ONTOLOGY, IMGT Databases, Tools, and Web Resources for Immunoinformatics - Immunoinformatics - page 8

been automatically annotated by the IMGT/Automat tool, with annotations being as
reliable and accurate as those provided by a human annotator.
1.4.1.3 Other IMGT ® IG and TR Sequence Databases
IMGT/PRIMER-DB (Folch, Bertrand, Lemaitre, and Lefranc 2004) is the
IMGT ® oligonucleotide primer database for IG and TR, created by LIGM, Mont-
pellier in collaboration with EUROGENTEC S.A., Belgium, on the Web since
February 2002 (http://www3.oup.co.uk/nar/database/summary/505). In March
2007, IMGT/PRIMER-DB contained 1864 entries and provides standardized
information on oligonucleotides (or Primers) and combinations of primers (Sets,
Couples) for IG and TR. These primers are useful for combinatorial library con-
structions, scFv, phage display, or microarray technologies. The IMGT Primer
cards are linked to the IMGT/LIGM-DB flat files, and to the IMGT Repertoire
(IMGT Colliers de Perles and Alignments of alleles of the IMGT/LIGM-DB
reference sequence used for the primer description). IMGT/PROTEIN-DB is a
new IMGT ® database related to immunoglobulin and T-cell receptor amino acid
sequences. The database will be available on the IMGT ® Web site in 2007.
1.4.1.4 IMGT ® MHC Sequence Databases
IMGT/MHC-DB comprises databases hosted at EBI and includes a database of
human MHC allele sequences, IMGT/MHC-HLA (or IMGT/HLA), developed by
Cancer Research, UK and maintained by the Anthony Nolan Research Institute
ANRI, London, UK, on the Web since December 1998, and a database of MHC
sequences from nonhuman primates IMGT/MHC-NHP, curated by the Biomedical
Primate Research Centre BPRC, The Netherlands, on the Web since April 2002
(Robinson, Waller, Parham, de Groot, Bontrop, Kennedy, Stoehr, and Marsh 2003).
1.4.2 IMGT ® Sequence Analysis Tools
The IMGT ® tools for the genetics approach comprise IMGT/V-QUEST (Lefranc
2003; Giudicelli, Chaume, and Lefranc 2004), for the identification of the V, D, and
J genes and of their mutations, IMGT/JunctionAnalysis (Yousfi Monod, Giudicelli,
Chaume, and Lefranc 2004) for the analysis of the V-J and V-D-J junctions which
confer the antigen receptor specificity, IMGT/Allele-Align for the detection of
polymorphisms, IMGT/Phylogene (Elemento and Lefranc 2003) for gene evolution
analyses, and IMGT/DomainDisplay for amino acid sequences.
1.4.2.1 IMGT/V-QUEST
IMGT/V-QUEST (V-QUEry and STandardization) is an integrated software for IG
and TR (Lefranc 2003, Giudicelli et al. 2004), used for the identification of the V, D,
and J genes and of their mutations. This tool is easy to use for the analysis of input
IG or TR germline or rearranged variable nucleotide sequences. IMGT/V-QUEST
results comprise the identification of the V, D, and J genes and alleles and the nucleotide