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Fig. 6. Overall scheme of recessive mutant screening at RIKEN. G1 and G2 mice are obtained
by the same scheme as shown in Fig. 5 for the dominant mutant screening. Recessive traits,
however, are concealed in G1 and G2. In order to detect recessive mutations, randomly chosen
G1 and its direct progeny of G2 are mated to reproduce G3. In some cases, females and males
of the same G2 litter are mated to obtain G3 offspring.
Bar-coding . More than 100 G1 mice are produced every week. They remain un-
der investigation until 78 weeks of age. As a result, at any given time approximately
8000 G1 mice are present in our facility. G0, G2, and G3 mice and control mice are
maintained in a specific pathogen-free (SPF) animal facility. A bar-coding system
that facilitates the identification of each mouse is crucial for the daily husbandry
work.
Local-Area Network (LAN). The mice must be protected from lethal infections by
pathogens. Consequently, physical material transfer including the use of pens and
papers are severely limited to maintain the SPF grade. Data transfer also requires no
or minimal writing and copying errors. To meet these requirements, the entire data
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