Environmental Engineering Reference
In-Depth Information
azoreductases is their requirement for expensive co-factors and the toxicity of the
aromatic compounds produced. This can be overcome using whole cells systems of
recombinant cells overproducing azoreductases and laccases as described for the
decolorization and detoxi
cation of model wastewater baths. The bacterial dye
decolorizing peroxidases are new biocatalysts with a high potential for the set-up of
bioprocesses considering the enlarged substrate spectrum exhibited, in particular
their high ef
ciency for the biotransformation of anthraquinonic dyes. Both
enzymes from P. putida and B. subtilis are interesting biocatalysts showing higher
rates of decolorization as compared to azoreductases and the laccases tested.
Moreover, we have shown the bene
ts of genetic engineering and evolutionary
approaches to modify enzymes and microorganisms with enhanced stability and
catalytic performance towards an ef
cient enzymatic treatment of dye-containing
wastewaters.
Acknowledgments The authors are especially thankful to Dr. Luciana Pereira for her contribu-
tion in the establishment of this line of research in the laboratory. Funding is acknowledged from
the project grants SOPHIED-FP6-NMP2-CT-2004-505899 and BIORENEW-FP6-2004-NMP-NI-
4/026456 from the European Commission and POCI/AMB/56039/2004, PTDC/AMB/64230/
2006, PTDC/BIO/72108/2006, PTDC/AGR-CFL/103840/2008 and Pest-OE/EQB/LA0004/2011
from Funda
çã
o para a Ci
ê
ncia e Tecnologia, Portugal.
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