Bacterial Enzymes and Multi-enzymatic Systems for Cleaning-up Dyes from the Environment - Microbial Degradation of Synthetic Dyes in Wastewaters - page 43

Table 1 Activities of PpDyP and BsDyP as compared to PpAzoR and CotA, using 2 mM of
anthraquinonic (disperse blue 1, reactive blue 5, acid blue 62 and reactive blue 19) or azo (mordant
black 9, acid black 194 and acid yellow 49) dyes as substrate (Santos et al. 2014 )
Substrates V max (U mg − 1 )

PpDyP BsDyP PpAzoR CotA

AQ dyes
Disperse blue 1 nd 0.6 ± 0.04

10 ± 3 3 ± 0.1

0.3 ± 0.01 Reactive blue 5 nd

11 ± 1 9 ± 1

Acid blue 62 nd 1.3 ± 0.9

9 ± 1 10 ± 0.1

Reactive blue 19 nd nd

9 ± 2 5 ± 0.2

Azo dyes
Mordant black 9 2 ± 0.1 1 ± 0.3

26 ± 2 4 ± 0.1

Acid black 194 3 ± 0.4 0.9 ± 0.2

12 ± 2 2 ± 0.1

Acid yellow 49 2 ± 0.3 2 ± 0.2

10 ± 1 3 ± 0.2

nd not detected

120

BsDyp
PpDyp

100

80

60

40

20

0

AQ dyes

Azo dyes

Fig. 14 Dye decolorization by the enzymes PpDyP (black bars) and BsDyP (white bars).
Decolorization was measured by HPLC after 24 h of reaction (adapted from Santos et al. 2014 )

or phenolic substrates tested, than the Bacillus enzyme. Moreover, PpDyP is able to
oxidise the high redox non-phenolic veratryl alcohol compound (1.4 V) in the
absence of redox mediators as DyPB from R. jostii and DyPs from C-D subfamilies