Environmental Engineering Reference
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times higher than that of Cr(III). Cr and Ni also reduced dry (DM) and fresh (FM)
mass production of S. alba and their adverse effect was stronger on FM than on
DM production. The root FM was reduced, similar as growth, more strongly than
that of shoots. This indicates a reduction in water uptake. Because tested metals
significantly reduced mainly water content in the shoots, it can be concluded that
Cr and Ni inhibited not only water absorption by the roots but also water transport
into the upper seedlings parts. Genotoxicity of Cr and Ni was determined as number
of chromosomal aberrations in V. sativa root tips and micronuclei frequency in the
pollen tetrades of Tradescantia paludosa . All tested metals activated a significant
increase in chromosomal aberration rate at applied IC 50 concentrations. The maxi-
mum of aberrations in anatelophase cells invoked Cr(VI). None of tested metals
significantly stimulated, in comparison with the control, micronuclei frequency in
pollen tetrades of T. paludosa and genotoxicity effect decreased in the same order
as for majority of observed parameters: Cr(VI) ≥ Ni(II) > Cr(III).
Acknowledgements This study was supported by the Grant of Ministry of Education, Science,
Research and Sport of the Slovak Republic VEGA 1/0098/14.
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