Biomedical Engineering Reference
In-Depth Information
Class
Assay category
Confi guration/principle
Metric
Advantages
Disadvantages
Under agarose
Migration over
rigid surface
and under gel
+
Direct observation
−
Specialized
of movement
Chemotactic
equipment
+
gradient longer
lived and
well-controlled
Multiple
+
simultaneous
gradients
In
/
ex vivo
methods
Complexity of
in vivo
environment utilized/
mimicked
Path or fi nal
location
+
Cell behavior
−
Cost of maintaining
different
in vivo
than
in vitro
animals or procuring
animal material
Transparent model
organisms
Path or fi nal
location
+
Direct observation
−
Cost of maintaining
of movement
Variety of genetic
animals
+
mutants
Aortic ring
Number and
distance cells
migrate out
of tissue into
surrounding gel
+
Native matrix and
−
3D imaging required
multiple cell types
present
Bridges
−
Variability in
specimens
+
in vivo
/
in
vitro
Matrigel plug
Cell invasion into
or migration out
of implanted
Matrigel plug
+
Simplicity
−
End point assay
−
Cost of maintaining
animals
