Biomedical Engineering Reference
In-Depth Information
Bioreactor
confi guration
Conditions applied
Signifi cant results
Rotational
- Small GAG penetration inside constructs
- Uniform GAG deposition in rotating
constructs
- Increased Young's and dynamic modulus
Oscillatory bi-directional
perfusion
- Tightly woven scaffolds yielded constructs
with higher moduli than more loosely
woven scaffolds (values similar to native
articular cartilage)
- Bioreactor treatment resulted in higher
total collagen content and more spatially
homogenous tissue
- TGF
β
, ITS+ Premix - necessary for
chondrogenic differentiation (optimal
without serum - resulted in higher GAG
content, higher GAG/DNA ratio, earlier
expression of collagen type II mRNA etc.)
Pneumatic
Compression-perfusion
- Stimulation of PP, PF, PA and PEG constructs
resulted in an increase in the unconfi ned
true compressive modulus by 32%, 45.4%,
33.6%, and 28.2% respectively
- Molecular composition and architecture
of the scaffold infl uences the
mechanotransduction
Rotational
Perfusion, BMP-6
- Higher values of GAG, collagen II and DNA
amounts in 100 ng BMP-6 loaded chitosan
scaffolds
- Differentiated ATDC5 cells preserved
chondrogenic phenotypes without
hypertrophic morphology - formed
neo-tissue matrix
Perfusion, periodic
medium fl ow reversal
- BMP-6 and in the combination with
TGF-
β
1 increased GAG and total collagen
production WHEN after FGF-2 pre-treatment
- Extended culture did not improve cartilage
development in PGA scaffolds
- Two-fold increase in cell density
Rotating wall
vessel
- Strength of the tissue grown on collagen
sponge reached 0.9 MPa (day 7) and
0.52MPa without the scaffold (day 21)
- Enhance the GAG content (2.5X more than
without scaffold)
Perfusion
Cyclic compression
- GAG and DNA levels constant
- Collagen II deposition enhanced by perfusion
- Collagen X deposition enhanced by cyclic
compression (also mechanical stiffness and
differentiation)
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Continued
)
