Biomedical Engineering Reference
In-Depth Information
Bioreactor
confi guration
Conditions applied
Signifi cant results
Hollow fi ber
Bovine hemoglobin
supplementation
(15 g/L)
- High cell mass in the extracapillary space
- Improved metabolic effi ciency, capacity for
drug metabolism
- Conserved albumin synthesis and ammonia
detoxifi cation
Perfusion seeding and
cultivation
- Increased expression of CYP3A4 and
UGT2B7 enzymes
- Increased albumin secretion
Flat
membrane
Radial-fl ow, oxygen
supplied via of
porcine RBCs
- Increase in oxygen consumption, adhesion,
albumin secretion and urea production
- Increased ammonia elimination and glucose
consumption
Flat
membrane
SF-3 medium, human
plasma and
complement-
inactivated human
plasma
- Organotypical double-gel (DG) culture is
superior to conventional collagen-coated
single-gel (SG) cultures
- DG - lower GOT, GPT and LDH levels - higher
cell viability in terms of stable albumin
secretion and urea synthesis
- Ammonia detoxifi cation similar in both systems
Crossed
hollow
fi ber
membrane
Separate delivery and
removal systems
using PES and
PEEK-WC
membranes
- Simulated concentration differed 7.5% for
albumin and 5% for urea with respect to
experimental data
- High rate of urea synthesis (28.7 μg/h 10
6
cells) and diazepam biotransformation
(high cytochrome P450 expression)
Pulsatile
bioreactor
system
Preconditioned with conti-
nuous fl ow increased
through 10 steps up
to 1, 2 and 3 L/min
- Complete endothelialization at 2L/min fl ow
rate
- Decrease in platelet aggregation
Controlled stretch via
cyclic distension
- Incrementally control-stretched (ICS)
valves are mechanically comparable to
native tissue in radial direction but not
in circumferencial (however better than
statically treated samples)
- ICS have 37% less collagen density than
native tissues
- Shows comparable mechanical properties
under pulmonary pressures
Organ-level
Cyclic fl exure and fl ow,
(bFGF) and ascorbic
acid-2-phosphate
(AA2P), pulmonary
conditions
- Supplemented media accelerated collagen
formation
- Increased collagen mass production
- Oscillatory surface shear stresses similar to a
tri-leafl et valve
- PGA/PLLA not elastomeric, not able to produce
aTEVC that duplicated native valve motion
(
Continued
)
