Biomedical Engineering Reference
In-Depth Information
9.4
Conclusion and Perspective
ORs allow high selectivity and human nose-like performance for the bioelectronic
nose. Therefore, the production of ORs or cells expressing ORs should be a critical
process for the development of the bioelectronic nose. Along with advances in the
production of ORs using heterologous cell systems, including bacterial, mamma-
lian and insect cells, ORs have been applied as very efficient sensor elements for
bioelectronic noses. However, the production of ORs still remains as a bottleneck.
To mimic the natural human nose more closely, multiplexed sensors functional-
ized with various different human ORs should be developed. There are about 390
functional OR genes that have been identified in the human genome, and the suit-
able conditions for their production will be different from each other. For practical
applications and successful commercialization, some technical issues, including
the stability, functional reproducibility and mass production, will be required to
be established. Therefore, more elaborate process for the production of olfactory
receptors, including expression, solubilization, purification, reconstitution and im-
mobilization of the sensor material, need to be further investigated.
Acknowledgments
This work was supported by the National Research Foundation of Korea
(NRF) grant funded by the Ministry of Science, ICT & Future Planning (No. 2013003890,
No. 2013055375).
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