Chemistry Reference
In-Depth Information
FIGURE 14.24
Formation of the pO
2
image (a), (b), and (c) phosphorescence intensity
images at different time delays with respect to the laser pulse. (d) Measurement and single
exponential decay lifetime (
t
) fit of the phosphorescence intensity at point A marked with the
cross in (a). (e) In vitro calibration of Oxyphor R2 at high concentration (4
10
5
M). (f) pO
2
image was obtained based on the probe calibration and lifetime values in each pixel. Arterial
pO
2
measured during the experiment was
106mmHg. Scale bar is 1mm [128].
brain [106,107,127]. As an illustration, image of pO
2
in the rat brain, obtained using
Oxyphor R2 and wide-field time-domain microscopy setup,
is
shown in
Figure 14.24 [128].
More recent developments include high-resolution functional pO
2
measurements
in the brain by way of confocal microscopy (Figure 14.25) [18].
FIGURE 14.25
(a) CCD image of cranial window in a rat with confocal angiogram overlaid
and region of functional activation identified in green. (b) Color-coded angiogram of micro-
vessels in rat somatosensory cortex, with identified locations for pO
2
measurement [18]. (See
the color version of this figure in Color Plates section.)
