Agriculture Reference
In-Depth Information
(Agarwal et al., 2008). Integration events were
confi rmed by both Southern and segregation
analysis, and were shown to be mainly single
copy. In an alternative approach, transformed
plants were obtained after piercing a region of the
embryonic apical meristem in imbibed seeds with
a needle that had been dipped in
Agrobacterium
inoculum (Supartana et al., 2006). Transforma-
tion was confi rmed in the T
1
generation by PCR,
Southern analysis, and by isolation and sequence
analysis of wheat DNA fl anking the T-DNA
insertion sites. If the effi ciency of the
in planta
transformation technologies can be enhanced,
they will provide a powerful tool for developing
functional genomics tools.
There is thus real potential for being able to
conduct large-scale structural and functional analy-
ses in wheat in the coming years. In addition to
having the sequence of all wheat genes available, the
wheat research community will have access to the
necessary functional genomics tools to elucidate
the function of the genes that are key to determin-
ing the agricultural importance of wheat. Detailed
knowledge about the action mechanisms in wheat
of genes identifi ed by QTL and association studies
as underlying specifi c traits, of genes shown in
model species to underlie particular pathways, and
of previously “unknown” genes annotated from
sequence data will provide a means for highly effi -
cient and targeted improvement of wheat.
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