Biomedical Engineering Reference
In-Depth Information
A
LM
E
B
F
C
D
FIB
15.6
Schematic presentation of the sample preparation chain. (A)
Fixation, dehydration and plastic embedding. (B) Dividing the
embedded tissue-implant block in half. (C) Mounting on a plastic
slide and sawing. (D) Grinding until a final thickness of 50 µm. (E)
Staining prior to light microscopic analysis. (F) Sputter coating with
gold prior to FIB sectioning for TEM analysis. LM is light microscopy.
2 mm
(a)
Titanium
Bone
2
m
m
(b)
15.7
(a) Light micrograph of the stained ground section. (b) SEM
micrograph of the FIB prepared TEM sample.
