Biomedical Engineering Reference
In-Depth Information
A
BADS
mPEG-NH
2
PLG NCA
mPEG
-
b
-
PPLG
Micellization
Crosslinking
bads
100
150
B
C
ac
a
d
b
80
100
60
40
50
20
0
0
0
0
20
30
40
50
60
70
80
10
-1
10
0
10
1
10
2
10
3
Time (h)
Fluorescence Intensity
Figure 15.5
(A) Preparation procedure of reduction-responsive nanogel. (B)
In vitro
release of DOX from (a) the DOX-loaded micelle, (b) nanogel without GSH, (c) nanogel
with 5.0 mM GSH and (d) nanogel with 10.0 mM GSH in PBS at pH 7.4, 37°C. (C) Flow
cytometric proi les of HeLa cells incubated with the DOX-loaded nanogel for 2 h: (a) cells
without pretreatment; (b) cells pretreated with 0.5 mM BSO; (c) cells pretreated with 10.0
mM GSH. Reproduced with permission from [77].
Moreover, Chen and coworkers have prepared the reduction-responsive
polypeptide nanogel through core crosslinking poly(ethylene glycol)-
block
-poly(γ-propargyl-L-glutamate) (PEG-
b
-PPLG) micelle by Cu(I)-
catalyzed Huisgens cycloaddition “click” reaction with bis(2-azidoethyl)
disuli de (BADS) (Figure 15.5A) [77]. h e DOX-loaded nanogel displayed
accelerated
in vitro
drug release in a reductive condition (Figure 15.5B)
and exhibited enhanced intracellular DOX release (Figure 15.5C) and
in
vitro
cellular proliferation inhibition toward HeLa cells with the pretreat-
ment of GSH.
15.2.3.3 pH and Reduction Dual-Responsive Polypeptide Nanogels
Of all the intelligent nanogels, multiple stimuli-responsive nanogels,
especially those responding to both pH and reduction, exhibited great
promise for targeting intracellular transportation of antitumor drugs.
