Biomedical Engineering Reference
In-Depth Information
when studying the biochemical parameters of an organism which is tre-
mendously important in medical practice.
13.2.2
Fiber Pptic Immune Biosensor Based on the Principle
of the “Evanescent” Wave
h e principle of non-emissive energy transfer between two l uorescent
labels, when one of them is belonging to Ag and the other one to Ab, may
be used for creating optical immune biosensors [3, 4].
13.2.2.1
Construction, Used Reagents and Measurements [5]
A schema for the proposed biosensor is given in Figure 13.2. Light from
the argon laser (1) spreads through the l at parallel plate (2) and semitrans-
parent mirror (9), and then falls on the entrance butt-end of the optrode
(10). h e l uorescent signal from the lateral optrode surface caused by the
evanescent wave was tunneled through the back of the optrode. h is sig-
nal is rel ected from the semi-transparent mirror (9), falling on the higher
ef ective beam splitter. One part of the rays (rel ected light) is directed to
the photoelectric cell with a maximum band-pass at 575 nm (semi wide
8 nm). h e other part of the rays (passed through 11) falls on the second
Figure 13.2
A. Scheme of optical immune biosensor based on the principle of the
“evanescent” waive for the simultaneous measurements of the presence of two analytes in
the solution (detailed explanation is in the text). B. Overall view of competitve principle
of analysis. Where: 1 - optrode, 2 - sample; 3, 4 - immobilized Ab to phenatoine and
lidocaine, respectively, 5 - phenatoine, 6 - phenatoine labeled by FITC, 7 - lidocaine, 8
- lidocaine labeled by R-Phy, 9-11 - l uorescent irradiation that arouses at the excitation
FITC and R-Phy, respectively (B).
