Biomedical Engineering Reference
In-Depth Information
thickness) on the surfaces of PS microbeads. h e specii c surface area of the
papain-imprinted beads was about 180m
2
g
-1
and its pore size was 31 nm.
h ese imprinted microbeads exhibit high recognition specii city and fast
mass transfer kinetics. h e specii city of these imprinted beads mainly
originates from the spatial ef ect of imprinted sites, because the protein-
imprinted sites were located at, or close to, the surface, the imprinted beads
have good site accessibility toward the template molecules. h e facility of
the imprinting protocol and the high recognition properties of imprinted
microbeads make the approach an attractive solution to problems in the
i eld of biotechnology.
11.3
Imprinted Materials at Nanoscale
11.3.1 Imprinted Nanoparticle
Recently Kobra
et al.
, reported the synthesis of nanoparticles of MIPs were
by precipitation polymerization method using glucose as a template mole-
cule [77]. Experimental data based on uniform design were analyzed using
artii cial neural network to i nd the optimal condition. h e results showed
that the binding ability of nanoparticles of MIPs prepared under optimum
condition was much higher than that of the corresponding non-imprinted
nanoparticles (NIPs).
Behbahani
et al.
, describes the preparation of new Pb(II)-imprinted
nanostructured polymeric particles using 2-vinylpyridine as a functional
monomer, ethylene glycol dimethacrylate as the cross-linker, 2,2'- azo-
bisisobutyronitrile as the initiator, diphenylcarbazone as the ligand, ace-
tonitril as the solvent, and Pb(NO
3
)
2
as the template ion, through bulk
polymerisation technique. h e prepared ion-imprinted nanostructured
polymer particles have an increased selectivity toward Pb(II) ions over
a range of competing metal ions with the same charge and similar ionic
radius. h is ion-imprinted polymer is an ei cient solid phase for extrac-
tion and preconcentration of lead ions in complex matrixes [78].
Forouzani
et al.
, have been reported nalidixic acid imprinted uniformly
sized polymers in the nanometer range by precipitation polymerization
using methacrylic acid (MAA) and methyl methacrylate (MMA) as func-
tional monomers at dif erent mole ratios [79]. h e ef ect of combination
of MAA-to-MMA on the morphology, binding, recognition and release
behaviors of the i nal particles were studied. h e produced polymers were
characterized by dif erential scanning calorimetry and their morphology
was precisely examined by scanning electron microscopy. A very uniform
