Biomedical Engineering Reference
In-Depth Information
Gold
electrode
TiO
2
-NH
2
AuNPs
Cysteamine
MIP with
template
After remove
template
Figure 11.31
h e simplii ed sketch for the fabrication process of the electrode [70].
humans through altering endocrine function. Huang
et al.
developed
a novel electrochemical sensor for sensitive and fast determination of
4-NP [70]. TiO
2
-NPs and AuNPs were introduced for the enhancement
of electron conduction and sensitivity. 4-NP-imprinted functionalized
AuNPs composites with specii c binding sites for 4-NP was modii ed
on electrode (Figure 11.31). Rebinding experiments were carried out to
determine the specii c binding capacity and selective recognition. h e
linear range was over the range from 4.80×10
−4
to 9.50×10
−7
mol L
−1
, with
the detection limit of 3.20×10
−7
mol L
−1
(S/N=3). h e sensor was success-
fully employed to detect 4-NP in real samples.
In another work, the preparation of CNTs functionalized with MIPs
for advanced removal of estrone has been reported by Gao
et al
[71].
CNTs@Est-MIPs nanocomposites with a well-dei ned core-shell struc-
ture were obtained using a semi-covalent imprinting strategy, which
employed a thermally reversible covalent bond at the surface of silica-
coated CNTs for a large-scale production (Figure 11.32). h e adsorption
properties were demonstrated by equilibrium rebinding experiments
and Scatchard analysis. h e results demonstrate that the imprinted
nanocomposites possess favourable selectivity, high capacity and fast
kinetics for template molecule uptake, yielding an adsorption capacity
of 113.5μmol/g. h e synthetic process is quite simple, and the dif erent
batches of synthesized CNTs@Est-MIPs nanocomposites showed good
reproducibility in template binding. h e feasibility of removing estro-
genic compounds from environmental water using the CNTs@Est-MIPs
nanocomposites was demonstrated using water samples spiked with
estrone.
