Biomedical Engineering Reference
In-Depth Information
Rebind
MWCNTs-AuNPs
Chitosan
Pre-polymer
Misture
Remove
GCE
MWCNTs-AuNPs
Chitosan
Monomer
Tyramine
Figure 11.29
h e preparation of the molecularly imprinted tyramine-sensing electrode [64].
Some nanoparticle-MWCNTs composites are also reported in the lit-
erature viz., Huang
et al.
developed a novel sensitive molecularly imprinted
electrochemical sensor for selective detection of tyramine by combination
of MWCNT-AuNP composites and chitosan [64]. MWCNT-AuNP com-
posites were introduced for the enhancement of electronic transmission
and sensitivity. Chitosan acts as a bridge for the imprinted layer and the
MWCNT-AuNP composites. h e MIP was synthesized using tyramine
as the template molecule, silicic acid tetracthyl ester and triethoxyphe-
nylsilane as the functional monomers (Figure 11.29). h e molecularly
imprinted i lm displayed excellent selectivity towards tyramine. Under the
optimum conditions, the current response had a linear relationship with
the concentration of tyramine in the range of 1.08×10
-7
to 1×10
-5
mol/L,
with a limit of detection 5.7×10
−8
mol/L. h e proposed sensor exhibited
excellent repeatability, which was better than the result from previous lit-
erature. h e relative standard deviation (RSD) of the repeated experiments
for tyramine (5 mmol/L) was 7.0%. Determination of tyramine in real
samples showed good recovery.
h e author also reported a molecularly imprinted electrochemical sen-
sor based on gold electrode decorated by β-cyclodextrin (CD) incorpo-
rated MWCNTs, AuNPs-polyamide amine dendrimer nanocomposites
(Au-PAMAM) and chitosan derivative (CSDT) for selective and convenient
determination of chlortetracycline (CTC) [65] and oxytetracycline (OTC)
[66]. h e electrochemical sensor was fabricated by stepwise modii cation
of cyclodextrin-CNTs composites (CD-MWCNTs) and Au-PAMAM onto
the gold electrode (Figure 11.30). h e layer of MIPs was the outer layer of
the electrochemical sensor. Herein, CSDT acted as functional monomer,
and CTC or OTC as the template molecule. CV and amperometry were
used to characterize the electrochemical behavior of the developed sensor.
h e linear range of the molecularly imprinted sensor was from 9.00×10
-8
to 5.00×10
-5
mol/L (from 0.0464 mg/kg to 25.767 mg/kg), with the LOD
