Biomedical Engineering Reference
In-Depth Information
BHb
DA
extraction
polymerization
rebinding
SiNW
Figure 11.23
Schematic of preparation of MIP for Bovine Hemoglobin (BHb) recognition [53].
Preparations of nanowire and/or i bers are less popular in the i eld of
imprinting compared to other nanostructures.
In 2012, Chen
et al.
employed silicon nanowires as the reinforcement
material in protein molecular imprinting with dopamine as the monomer
and BHb as the template molecule (Figure 11.23). In the experiments, the
imprinted nanowires showed fast adsorption kinetics (took up 75% of the
equilibrium amount during only 5min), signii cant selectivity and large
binding capacity (213.7 mg g
-1
) for the template protein. Furthermore, the
stability and regeneration were also investigated, which indicated that the
imprinted nanowires had outstanding reusability.
Electrospinning method was also employed for the preparation of
imprinted nanoi bers. For example, Kim
et al.
prepared an estrone imprinted
polyimide nanoi ber mat using an electrospinning method [54]. h e diamine
monomer-template complex was synthesized by the reaction of the diamine
monomer having an isocyanate group and estrone (template) having a
phenol moiety, in which the template was attached to the monomer via a
thermally reversible urethane bond. A poly(amic acid) was synthesized by
polymerization of the diamines (1:19 mole ratio of the diamine monomer-
template complex to 4,4 -oxydianiline) and pyromellitic dianhydride in
N,N-dimethylformamide and the reaction solution was used for electrospin-
ning (Figure 11.24). h e poly(amic acid) i bers were thermally imidized and
then heated in 1,4-dioxane in the presence of water to remove the template
molecules. h e imprinted polyimide nanoi bers showed the specii c recogni-
tion ability and fast kinetic adsorption for estrone.
11.2.1.8
Carbon Nanotubes (CNTs)
Carbon nanotubes can be visualized as a sheet of graphite that has been
rolled into a tube. Unlike diamond, where a 3-D diamond cubic crystal
