Biomedical Engineering Reference
In-Depth Information
11.1.2
h
e MIP 'Rule of Six'
1. Never use the analyte as a template unless there is absolutely
no alternative.
2. Make rational choices about which regions of an analyte
are likely to command the best types of interaction in a low
dielectric medium (organic solvent) and then incorporate
these elements in an analog of the analyte molecule.
3. Select monomers that are likely to form strong interactions
in the chosen solvent (e.g., Brönsted acids or bases/H-donors
or acceptors/nonpolar groups, etc.) - this will increase capac-
ity and inl uence homogeneity of the binding cavities.
4. Choose templates and monomers that will be soluble in the
porogenic solvent to be used in the polymerization - this
may seem obvious but it sometimes requires carrying out
solubility tests.
5. Ensure as far as possible that the template-monomer mix-
ture is stable and does not undergo side reactions under the
polymerization conditions.
6. Consider the nature of the matrix from which the analyte
will eventually be extracted when selecting the cross-linking
monomer- a range of di- or tri-unsaturated cross-linking
monomers (e.g., vinylic, acrylic, methacrylic, acrylamide,
etc.) with varying chemistries are available to create the
porous organic network material.
Keeping in view the popularity and wide applications of MIP and/
or MIP-based materials, we like to basically focus on their demerits and
improvement approach in this chapter.
11.1.3
Downsides of “h
e Imprinted Materials”
According to a very famous quotation “some times the good things became
bad for their selves”, just like that the major drawback of imprinted materi-
als are the ai nity of the imprinted cavities for the template which make
their removal very hard. So, the major problem associated with MIP is
washing of template from their binding sites. Because, if some template
molecules there are remaining inside the MIPs, fewer cavities will be avail-
able for rebinding, which decreases ei ciency of imprinted material [9].
However, there are some other problem also ascends due to the incomplete
removal of template like Figure 11.3:
