Label-Free Biochips - Advanced Biomaterials and Biodevices - page 296

Amperometric Potentiometric (pH)

Potentiometric Impedimetric

Figure 8.2 Silicon-based amperometric, potentiometric and impedimetric sensor all
integrated on a single chip (Nakazato, 2013).

A B

S D

15-20 m Hypoxic buffer

PC12 cell

Ca 2+

Nu

Dopamines

DAQ

S D

Lock-in
amplifier

3 m
MPC aptamer/SiNW-FET

C

Aptamer

MBS

~ 2nn

3'

5'

APTMS PTMS

~ 1nn

SiNW SiNW SiNW SiNW

Figure 8.3 (A) Illustration of the experimental setup of a DNA-aptamer- modii ed MPC
SiNW-FET device for detecting exocytotic DA under hypoxic stimulation from living
PC12 cells. (B) Optical microscopy image of an MPC SiNW-FET device. S = source;
D = drain. (C) Procedure for immobilization of the DNA-aptamer on an MPC
SiNW- FET. Abbreviations: APTMS, (3-aminopropyl)trimethoxysilane; PTMS,
propyltrimethoxysilane; MBS, 3-maleimidobenzoic acid N-hydroxysuc- cinimide ester
(Li et al. , 2013).