Chemistry Reference
In-Depth Information
12
APPLiCAtion oF
d
- AnD
f
-bLoCk FLUoresCent
CeLL iMAging Agents
Michael P. Coogan
Department of Chemistry, Lancaster University, Lancaster, UK
Simon J. A. Pope
School of Chemistry, Cardiff University, Cardiff, UK
AbbreviAtions
bpy
2,2′-bipyridine
CHo
Chinese Hamster Ovarian
Cyclen
1,4,7,10-tetraazacyclododecane
Do3A
1,4,7,10-tetraazacyclododecane-1,4,7-triacetic acid
DotA
1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid
DPPZ
dipyrido-[3,2-a:2′,3′-c]-phenazine
FLiM
Fluorescence Lifetime Imaging Microscopy
LMCt
Ligand to Metal Charge Transfer
MLCt
Metal to Ligand Charge Transfer
nADPH
Nicotinamide adenine dinucleotide phosphate
nHC
Nitrogen Heterocyclic Carbene
nir
Near Infra Red
phen
1,10-phenanthroline
PnA
Peptide Nucleic Acid
ppy
2-phenylpyridine
pybz
2-pyridyl-benzimidazole
ros
reactive oxygen species
terpy
2,2′;6′,2′′-terpyridine
trLM
Time-resolved Luminescence Microscopy
U.v.
Ultra Violet
12.1
introDUCtion
Because the previous chapter focused on the principles of fluorescent techniques (e.g., PET, FRET, FLIM) for the
in vitro
and
in vivo
studies of interactions among biomolecules and the chemosensing of important analytes, whilst this chapter will focus
heavily on applications in fluorescence microscopy, a short overview of the techniques involved may be useful. Fluorescence
