Biomedical Engineering Reference
In-Depth Information
nce
ori
Amp
R
Express fusion protein in
E. coli
Lyse cells
biotin
tag
Xa
gene product
Affinity chromatography with streptavidin column
Streptavidin
biotin
tag
Xa
gene product
Streptavidin
Elute by adding excess biotin
biotin
tag
Xa
gene product
Cleave fusion protein with factor Xa
Fig. 5.14
Purification of a cloned gene
product synthesized as a fusion to the
biotin carboxylase carrier protein (tag).
See text for details.
biotin
tag
gene product
