Basic biology of plasmid and phage vectors - Principles of Gene Manipulation - page 54

pUC19

1
THR
ACC

2
MET
ATG

3
ILE
ATT

4
THR
ACG

1
pro
CGA

2
ser
AGC

3
leu
TTG

4
his
CAT

5
ala
GC C

6
cys
TGC

7
arg
AGG

8
ser
TCG

9
thr
AC T

10
leu
CTA

11
glu
GAG

12
asp
GAT

13
pro
CC C

14
arg
CGG

15
val
GTA

16
pro
CCG

17
ser
AGC

18
ser
TCG

5
ASN
AAT

6
SER
TC A

7
LEU
CTG

8
ALA
GCC

ATG

Hin d III Sph I Psf I Sal I

Acc I
Hin c II

Xba I Bam H I K pn I Sst I Eco R I Hae III

Xma I
Sma I

1
THR
ACC

2
MET
ATG

3
ILE
ATT

4
THR
ACG

5
ASN
AAT

6
SER
TCG

1
ser
AGC

2
ser
TCG

3
val
GTA

4
pro
CC C

5
gly
GGG

6
asp
GAT

7
pro
CC T

8
leu
CTA

9
glu
GAG

10
ser
TCG

11
thr
AC C

12
cys
TGC

13
arg
AGG

14
his
CAT

15
ala
GC A

16
ser
AGC

17
leu
T T G

18
ala
GCA

7
LEU
CTG

8
ALA
GCC

pUC18

ATG

Eco R I Sst I Kpn I Bam H I Xba I Sal I

Acc I
Hin c II

Pst I Sph I Hin d III Hae III

Xma I
Sma I

1
THR
ACC

2
MET
ATG

3
ILE
ATT

4
THR
ACG

1
pro
CCA

2
ser
AGC

3
leu
TTG

4
gly
GGC

5
cys
TGC

6
arg
AGG

7
ser
TCG

8
thr
AC T

9
leu
CTA

10
glu
G A G

11
asp
GAT

12
pro
CC C

13
arg
CGG

14
ala
GCG

15
ser
AGC

16
ser
TCG

5
ASN
AAT

6
SER
TC A

7
LEU
CTG

8
ALA
GCC

pUC13

ATG

Hin d III Pst I Sal I

Acc I
Hin c II

Xba I Bam H I Sst I Eco R I Hae III

Xma I
Sma I

1
THR
ACC

2
MET
ATG

3
ILE
ATT

4
THR
ACG

5
ASN
AAT

6
SER
T C G

1
ser
AGC

2
ser
TCG

3
pro
CCC

4
gly
GGG

5
asp
GAT

6
pro
CC T

7
leu
CTA

8
glu
GAG

9
ser
TCG

10
thr
AC C

11
cys
TGC

12
ser
AG C

13
pro
CCA

14
ser
AGC

15
leu
TTG

16
ala
GCA

17
LEU
CTG

18
ALA
GCC

pUC12

ATG

Eco R I Sst I Bam H I Xba I Sal I

Acc I
Hin c II

Pst I Hin d III Hae III

Xma I
Sma I

1
THR
ACC

2
MET
ATG

3
ILE
ATT

4
THR
ACG

1
pro
CCA

2
ser
AGC

3
leu
TTG

4
ala
GCT

5
ala
GCA

6
gly
GGT

7
arg
CGA

8
arg
CGG

9
ile
ATC

10
pro
CC C

11
gly
GGG

5
ASN
AAT

6
SER
TC A

7
LEU
CTG

8
ALA
GCC

pUC9

ATG

Hin d III Pst I Sal I

Acc I
Hin c II

Bam H I Eco R I Hae III

Xma I
Sma I

1
THR
ACC

2
MET
ATG

3
ILE
ATT

4
THR
ACG

5
ASN
AAT

6
SER
TC C

1
arg
CGG

2
gly
GGA

3
ser
TCC

4
val
GTC

5
asp
GAC

6
leu
CTG

7
gln
CAG

8
pro
CCA

9
ser
AGC

10
leu
TTG

11
ala
GCA

7
LEU
CTG

8
ALA
GCC

pUC8

ATG

Eco R I B am H I Sal I

Acc I
Hin cII

Pst I Hin d III Hae III

Xma I
Sma I

1
THR
ACC

2
MET
ATG

3
ILE
ATT

4
THR
ACG

5
ASN
AAT

1
ser
TC C

2
pro
CCG

3
asp
GAT

4
pro
CCG

5
ser
TCG

6
thr
AC C

7
cys
TGC

8
arg
AGG

9
ser
TCG

10
thr
ACG

11
asp
GAT

12
pro
CCG

13
gly
GGG

14
asn
AAT

6
SER
TC A

pUC7

ATG

Eco R I Bam H I Sal I

Acc I
Hin c II

Pst I Sal I

Acc I
Hin c II

Bam H I Eco R I

Bsm B I 2683
Eco O 109 I 2674
Aat II 261 7
Bci V I 2542
Ssp I 2501
Eco 57 I 2381
Acl I 2297
Xmn I 2294

Bsm B I 51
Drd I 91

Nde I 183
Bst AP I 179
Nar I - Kas I - Sfo I 235
Bgl I 245
Fsp I 256
Pvu I 276
Pvu II 306

Polylinker
c loning sites
396-454

Bcg I 2215
Sca I 2177

Pvu I 2066
Ava II 2059

Pvu II 628
Tfi I 641
Sap I 683

Bsr D I 1935
Acl I 1924
Fsp I 1919
Ava II 1837
Bgl I 1813
Bpm I 1784
Bsr F I 1779
Bsa I 1766
Bsr D I 1753

Tfi I 781
Afl III 806

Drd I 908

Bci V I 1015

Ahd I 1694

Eco 57 I 1333 Alw N I 1217

Fig. 4.9 Genetic maps of some pUC plasmids. The multiple cloning site (MCS) is inserted into the lacZ gene but does not interfere
with gene function. The additional codons present in the lacZ gene as a result of the polylinker are labelled with lower-case letters.
These polylinker regions (MCS) are identical to those of the M13 mp series of vectors (see p. 63).