Biomedical Engineering Reference
In-Depth Information
EcoR
I
- Apo
I
4359
Cla
I
- Bsp
D
I
23
Hin
d
III
29
Eco
R V
185
Nhe
I
229
Bam
H
I
375
Sgr
A
I
409
Ban
II
471
Ban
II
485
Dsa
I
528
Sph
I
562
Eco
N
I
622
Sal
I
651
Hinc
II
651
Acc
I
651
Psh
A
I
712
Aat
II
4284
Bsr
B -
Bci
V
I
4209
Ssp
I
4168
Ear
I
4155
Eco
57
I
4054
Xmn
I
3961
Hin
c
II
3905
Sca
I
3844
Pvu
I
3733
Bsr
D
I
3608
Pst
I
3607
Ase
I
3537
Eag
I
939
Bsa
I
3433
Bsr
D
I
3420
Nru
I
972
Bst
AP
I
1045
Ahd
I
3361
†
Bsp
M
I
1063
Pfl
M
I
1315
Bsm
I
1353
Sty
I
1369
Pfl
M
I
1364
Ava
I
- Bso
B
I
1425
Ppu
M
I
1438
Msc
I
1444
Dsa
I
1447
Ppu
M
I
1480
Bsg
I
1656
BspE
I
1664
Bsa
B
I
1668
Eco
57
I
3000
Alw
N
I
2884
Bci
V
I
2682
Drd
I
2575
Afl
III
- Bsp
LU11
I
2473
Bsr
B
I
2408
Ear
I
2351
Sap
I
2350
Nde
I
2295
BstAP
I
2291
Bst
Z17
I
- Acc
I
2244
Bsa
A
I
2225
Xmn
I
2029
Pvu
II
2064
Bsm
B
I
2122
Drd
I
2162
Pfl
F
I
- Tth111
I
2217
Fig. 4.8
Restriction map of plasmid pBR322 showing the location and direction of transcription of the ampicillin (Ap) and
tetracycline (Tc) resistance loci, the origin of replication (
ori
) and the Col E1-derived Rop gene. The map shows the restriction
sites of those enzymes that cut the molecule once or twice. The unique sites are shown in bold type. The coordinates refer to the
position of the 5′ base in each recognition sequence with the first T in the
Eco
RI site being designated as nucleotide number 1. The
exact positions of the loci are: Tc, 86 -1268; Ap, 4084 -3296; Rop, 1918 -2105 and the origin of replication, 2535.
