Biomedical Engineering Reference
In-Depth Information
130 kb
Wild type AcMNPV DNA
(replication competent)
Deleted polyhedrin gene + flanking
regions (replication defective).
Transfer vector - insert foreign
gene in polyhedrin sequence.
Transfect with derivative AcMNPV DNA
Foreign gene
P
Homologous recombinant
Recombinant AcMNPV DNA
(replication competent)
Fig. 10.7
Procedure for the generation of recombinant baculovirus vectors.
