Environmental Engineering Reference
In-Depth Information
taBle 4.1 (continued)
cellulase, hemicellulase and cell Wall modifying enzymes expressed in Plants
expression
strategy/
targeting
activity assay/
temperature and ph
optima
enzyme/
source
native or
modified
host/tissue/
Phenotype
Promoter type/
source
maximum
yield
references
XynC xylanase/oleosin
fusion
Neocallimastix patriciarum
Native
Canola/Seed
Normal
Stable nuclear/
Oil bodies
Tissue specific/
Oleosin promoter
2000 U/kg
seed
RBB-xylan
40°C and pH 5.5
Liu et al.
1997
XylII xylanase
Trichoderma reesei
Native
Arabidopsis
/Leaves
Normal
Stable nuclear/
Cytosol
Chloroplast
Peroxisome
Chloroplast
and
Peroxisome
Light responsive/
Alfalfa RbcSK-1A
1.2% TSP
3.0% TSP
1.7% TSP
4.8% TSP
Reducing sugars released
from xylan
Hyunjong
et al. 2006
XynII xylanase
Trichoderma reesei
Native
Arabidopsis
/Leaves
Normal
Stable nuclear/
Cytosol
Chloroplast
Constitutive/
CaMV 35S
1.4% TSP
3.2% TSP
Reducing sugars released
from xylan
Bae et al.
2008
XynB xylanase
Streptomyces
olivaceoviridis
Native
Potato/Leave
Normal
Stable nuclear/
Cytosol
Constitutive/
Double CaMV
35S
5%
Reducing sugars released
from xylan
Yang et al.
2007
XynZ xylanase
Clostridium
thermocellum
Native
Tobacco/Leaves
Normal
Stable nuclear/
Apoplast
Constitutive/
CaMV 35S
4.1% TSP
Reducing sugars released
from xylan
Herbers
et al 1995
XylD xylanase CD
Ruminococcus flavefaciens
Native
Tobacco/Leaves
Normal
Stable nuclear/
Apoplast
Constitutive/
CaMV 35S
NR
Reducing sugars released
from xylan
Herbers
et al. 1996
Glucanase
Hybrid β-(1-3,1-4)
glucanase Baccillus sp.
Codon optimized
Barley/Seed
Normal
Stable nuclear/
Vacuole
Developmentally
regulated and
tissue specific/
Barley D hordein
5.4% TSP
Zymogram
Horvath
et al. 2000
