Environmental Engineering Reference
In-Depth Information
taBle 4.1 (continued)
cellulase, hemicellulase and cell Wall modifying enzymes expressed in Plants
expression
strategy/
targeting
activity assay/
temperature and ph
optima
enzyme/
source
native or
modified
host/tissue/
Phenotype
Promoter type/
source
maximum
yield
references
Cel6A endoglucanase/
Thermobifida fusca
Native
Tobacco/Leaves
Normal
Chloroplast
transformation/
Chloroplast
Constitutive/
plastid rrn16
2% TSP
CMC
Yu et al.
2007
DB-Cel6A endoglucanase/
Thermobifida fusca
Native
Tobacco/Leaves
Normal
Chloroplast
transformation/
Chloroplast
Constitutive/
Plastid rrn16
10.7% TSP
CMC% T
Gray et al.
2008
CelA/Cel6G endoglucanse/
Neocallimastix
patriciarumPiromyces spp.
Codon optimized
Barley/Seed endosperm
Normal
Stable nuclear/
Cytosol
Developmentally
regulated and
tissue specific/
Rice GluB-1
1.5% TSP
AZCL-barley glucans
AZCL-hydroxyethyl
cellulose
Xue et al.
2003
CelD endoglucanase
Clostridium thermocellum
Native
Tobacco/Leaves
Normal
Chloroplast
transformation/
Chloroplast
Constitutive/
psbA
4900 U/g FW
leaf
CMC
60C and pH 6
Verma et al.
2010
exoglucanases
Cel6B exoglucanase/
Thermobifida fusca
Native
Tobacco/Leaves
Normal
Chloroplast
transformation/
Chloroplast
Constitutive/
plastid rrn16
3% TSP
BMCC
Yu et al.
2007
CelO exoglucanase/
Clostridium thermocellum
Native
Tobacco/Leaves
Normal
Chloroplast
transformation/
Chloroplast
Constitutive/
psbA
442 U/mg TSP
Verma et al.
2010
β-D-glucan (1%)
CBHI exoglucanase/
Trichoderma reesei
Codon optimized
first 40 codons
Corn/Seed
Normal
Stable nuclear/
ER Apoplast
Vacuole
Seed specific/
Globulin 1
17.8% TSP
16.3% TSP
0% TSP
MUCase
Hood et al.
2007
CBHI exoglucanase/
Trichoderma reesei
Native
Tobacco/Leaves
Normal
Stable nuclear/
Native signal
Constitutive/
CaMV 35S
0.11% TSP
MUCase
Dai et al.
1999
